PurKine™ Endotoxin Removal Kit (Polymyxin B) - 1 mL
| Applications notes | PurKine™ Endotoxin Removal Resin has been designed to reduce endotoxin levels in protein samples by ≥99% while maximizing protein recovery. The Resin consists of 90μm beads of cross-linked 4% agarose, to which modified polymyxin B (PMB) has been coupled. Tests confirm that performance equals or exceeds popular polymyxin B (PMB) resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. |
| Alternative | Endotoxin Removal resin; polymyxin B resin; polymyxin B |
Product Properties
| Formulation | Liquid solution with 50% slurry in 20% ethanol. |
| Kit components | • PurKine™ Endotoxin Removal polymyxin B Packed Column • Regeneration buffer(10X) • Equilibration buffer(10x) |
| Features & Benefits | • High capacity—More than 2,000,000 EU/mL to eliminate >99% of endotoxins. • High performance—Protein recovery is more than 85%.Reducing final EU concentration to 0.1 EU/mL • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits. |
| Storage instructions | Stable for one year at 2-8°C from date of shipment. Do not freeze. |
| Shipping | Blue ice |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | PurKine™ Endotoxin Removal Resin is effective for reducing endotoxin levels in protein samples. Endotoxins, also known as lipopolysaccharides or LPS, are cell membrane components of Gram-negative bacteria (e.g. E. coli). Endotoxins strongly influence transfection of DNA into primary cells and sensitive cultured cells, and increased endotoxin levels lead to sharply reduced transfection efficiencies. The Resin consists of 90μm beads of cross-linked 4% agarose, to which modified polymyxin B has been coupled. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Endotoxin Removal Resin is also available as prepacked spin column and kit formats. |
| Alternative | Endotoxin Removal resin; polymyxin B resin; polymyxin B |