Panspecies Carnitine (VB20, Vitamin B20) ELISA Kit - 48 wells plate

Product name: CNT (Carnitine) ELISA Kit

Reactivity: General

Alternative Names: VB20; Vitamin B20

Assay Type: Competitive Inhibition

Sensitivity: 4.84 ng/mL

Standard: 1000 ng/mL

Range: 15.63-1000 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2h

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CNT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CNT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CNT in the samples is then determined by comparing the OD of the samples to the standard curve.