Human CTXII(Cross Linked C-Telopeptide Of Type II Collagen) ELISA Kit - 96 wells plate
| Product name: | Human CTXII(Cross Linked C-Telopeptide Of Type II Collagen) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | CTX-II; CTX2 |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 52.3 pg/mL |
| Standard: | 10000 pg/mL |
| Detection Range: | 156.25-10000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Metabolic pathway;Bone metabolism;Rheumatology; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CTXII protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTXII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTXII in the samples is then determined by comparing the OD of the samples to the standard curve. |