Mouse Anti-Sm Ig's (total (A+G+M) Quantitative ELISA Kit - 96 wells plate

The Mouse Anti-Sm Total Ig ELISA Kit is an im munoassay suitable for quantifying or titering total antibody activity (IgG, IgA and IgM) specific for Sm (Smith) antigen in serum or plasma. Other biological fluids, including tissue culture medium, may be validated for use

Antibodies reactive with autologous nuclear components, such

as DNA and histones, can represent an autoim

mune basis for pathological conditions such as systemic lupus erythematosis

(SLE) in humans. In mice homozygous for the

lymphoproliferation spontaneous mutation (Faslpr), a systemic

autoim munity develops with age which includes elevated levels

of anti-dsDNA and other anti-nuclear antibodies (ANA), including

anti-Sm (Smith antigen), a non-histone nuclear protein

associated with ribonucleoprotein particles and having a role in

post-translational pre-messenger RNA processing.

The expanded use in the drug industry of biological modifiers has

been associated with production of autoantibodies, of which

mice, and possibly also other hosts such as humans and

monkeys, are susceptible. A prototype disease in mice is lupus

caused by the drug minocycline, with elevated anti-dsDNA

among other autoantibodies and pathological conditions.

Recent investigations have focused on the role of innate im

mune mechanisms, including Toll-like receptors (TLRs) and TH2

im munity, responding to the damage-associated molecular

patterns of dying cells, as underlying cause of autoim

munity; these may be induced by drugs, including vaccines and

adjuvants, with aging, or with other health conditions.