AAV-2 CMV Expression System - 1 kit

• Complete Expression System contains packaging plasmids, expression vector and GFP control vector for packaging of recombinant AAV serotype 2
• pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
• Cloning capacity of expression vector = 3 kb

The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells.

Recent Product Citations

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3. Kuwabara, Y. et al. (2020). Lionheart LincRNA alleviates cardiac systolic dysfunction under pressure overload. Commun Biol. 3(1):434. doi: 10.1038/s42003-020-01164-0.
4. Okubo, Y. et al. (2019). Store-operated Ca2+ entry-dependent Ca2+ refilling in the endoplasmic reticulum in astrocytes. Biochem Biophys Res Commun. pii: S0006-291X(19)32326-5. doi: 10.1016/j.bbrc.2019.12.006.
5. Robert, M.A. et al. (2018). Gene Transfer of ZMapp Antibodies Mediated by Recombinant Adeno-Associated Virus Protects Against Ebola Infections. Hum Gene Ther. 29(4):452-466. doi: 10.1089/hum.2017.101.