HaCaT cells (In vitro spontaneously transformed keratinocytes from normal skin) - 1 vial

Origin: In vitro spontaneously transformed keratinocytes from histologically normal skin.

Species:Homo sapiens

Tissue: Skin

Morphology: Keratinocyte

Properties: Adherent monolayer

Patient: Male, Caucasian, 62 years of age.

Medium: Optimized DMEM (C0003-02) + 10% FBS

Subculture: Recommended 1:5 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C

Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level: I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

Hepatitis C: Negative

DNA Profile (STR):

Amelogenin: X,X

CSF1PO: 9,11

D13S317: 10,12

D16S539: 9,12

D5S818: 12

D7S820: 9,11

THO1: 9.3

TPOX: 11,12

vWA: 16,17

Related cells by Organ:

A431 cells

SK-Mel-28 cells

UACC-62 cells

C-32 cells

HaCaT cells

HaCaT Cells References:

1. Nakai, K., et al. J Dermatol Sci, 55: 170-178, 2009.

2. Markopoulou, S. et al. Febs J, 276: 3784-3799, 2009.

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4. Mohammad H, et al. PLoS One. 2015 Nov 4;10(11):e0142321. PMID: 26536129

5. Aijaz A,et al. Tissue Eng Part A. 2015 Nov;21(21-22):2723-2732. PMID: 26239745

6. Crochiere M, et al. BMC Cancer. 2015 Nov 17;15:910. PMID: 26573568

7. Duong DT, et al. Sci Rep. 2016;6:24952. doi: 10.1038/srep24952. PMID: 27117225

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9. Ahmed Al-Kattan, et al. RSC Adv. 2017;7:31759. DOI:10.1039/c7ra02255g

10. Jensen GS, et al. Clin Cosmet Investig Dermatol. 2016;9:357-366. PMID: 27789968

11. Mehta SB, et al. Proc Natl Acad Sci U S A. 2016;113(42):E6352-E6361. PMID: 27679846

12. Aksoy P, et al. PLoS One. 2017 Jan 17;12(1):e0170158. doi: 10.1371/journal.pone.0170158. PMID: 28095444

13. An X, el al. Oncotarget. 2017 Oct 12;8(53):90730-90747. doi: 10.18632/oncotarget.21817. PMID: 29207600

14. de Freitas LM, et al. BMC Cancer. 2017;17(1):123. doi: 10.1186/s12885-017-3075-1. PMID: 28187758

15. Gabbott CM, Sun T. Int J Mol Sci. 2018 Jan 28;19(2). pii: E388. doi: 10.3390/ijms19020388. PMID: 29382087

16. Lin-Shiao E, et al. Genes Dev. 2018 Jan 15;32(2):181-193. doi: 10.1101/gad.306241.117. PMID: 29440247