Human NEFL (Neurofilament, Light Polypeptide) Sandwich ELISA Kit - 96 wells plate
| Product name: | Human NEFL(Neurofilament, Light Polypeptide) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein |
| Assay Type: | Sandwich |
| Sensitivity: | 6.5 pg/mL |
| Standard: | 1000 pg/mL |
| Detection Range: | 15.63-1000 pg/mL |
| Sample Type: | serum, plasma, cerebrospinal fluid and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Neuro science; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NEFL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEFL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NEFL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEFL in the samples is then determined by comparing the OD of the samples to the standard curve. |