Anti-RFP Polyclonal Antibody - 300 ug

Other Names: Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Other Names: Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

Concentration: 3 mg/ml

Antibody Type: Primary

Target Type: anti-RFP

Accession Number: N/A

Host Species: Goat

Immunogen Species: Purified recombinant RFP peptide produced in E. coli.

Immunogen Type: Recombinant protein

Immunogen: Purified recombinant RFP peptide produced in E. coli.

Isotype: IgG

Clonality: Polyclonal

Confirmed Reactive Species: Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato

Specificity Detail: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Buffer: PBS, 20% glycerol and 0.05% sodium azide

Purification: Epitope affinity purified

Conjugation: Unconjugated

Format: PBS, 20% glycerol and 0.05% sodium azide

Application: WB, IF, IHC-P, IHC-F, IEM

Concentration/Dilution: WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Storage: Store at -20 C for long-term storage. Store at 2-8 C for up to one month.

References: 

1. D Jiang, D Correa-Gallegos, S Christ, et al. Nature Cell Biology 2018 Apr. PMID:29593327, 

2. Tian DS, Peng J, Murugan M, et al. J Neurosci. 2017 Aug. PMID:28716963, 

3. Semerci F, Choi WT, Bajic A, et al. Elife. 2017 Jul 12. PMID:28699891