Guinea pig (Cavia) Growth hormone (GH) ELISA Kit - 96 wells plate

This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double

antibody sandwich technology to assay the Guinea pig Growth hormone (GH). Add Growth

hormone(GH)to the wells, which are pre-coated with Growth hormone (GH) monoclonal

antibody and then incubate. After that, add anti GH antibodies labeled with biotin to unite

with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after

incubation and washing. Add substrate A and B. Then the solution will turn blue and change

into yellow with the effect of acid. The shades of solution and the concentration of Guinea pig

Growth hormone (GH) are positively correlated.

ASSAY RANGE: 1pg/ml - 200pg/ml

SENSITIVITY: 0.39pg/ml

ASSAY PROCEDURE SUMMARY:

1. Prepare all reagents, samples and standards;

2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.

3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at

37 ℃; for color development.

4. Add stop solution

5. Read the OD value within 10minutes.

6. Calculate.