X-gal (5-Bromo-4-chloro-3-indolyl-β-galactopyranoside) is the most widely used chromogenic substrate for β-galactosidase. Enzyme hydrolysis of the substrate yields a dark blue colored precipitate (maximum absorbance 615 nm).
- White solid soluble in DMSO
- Store desiccated at -20°C and protect from light
- C14H15BrClNO6
- MW: 408.6
- [7240-90-6]