Rat Myoglobin ELISA kit - 96 wells plate
INTRODUCTION:
Myoglobin is a 17 kDa heme protein expressed in cardiac and skeletal muscle. After cardiac injury, it is one of the first biomarkers to rise above normal levels. In humans, it increases above baseline within 2-4 hours, peaking at 9-12 hours, and returning to baseline within 24-36 hours. In the absence of skeletal muscle injury, it can used as a biomarker for cardiac muscle injury. Similarly, in the absence of cardiac damage, myoglobin can be used as a biomarker of skeletal muscle injury.
PRINCIPLE OF THE ASSAY:
The assay uses a monoclonal myoglobin antibody for solid phase immobilization (microtiter wells) and horseradish peroxidase (HRP) conjugated polyclonal myoglobin antibody for detection. Standards and diluted samples are co-incubated with HRP conjugate in the microtiter wells for one hour. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If myoglobin is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of myoglobin is proportional to absorbance and is derived from a standard curve.