Recombinant Staphylococcus aureus Panton-Valentine Leukocidin (PVL) F subunit (LukF-PV), Tag-free - 100ug

Description: Purified, recombinant Staphylococcus aureus Panton-Valentine Leukocidin (PVL) F subunit (LukF-PV). The theoretical molecular weight of the protein is 34,580 Daltons.

Storage: 2-3 weeks at -20°C, -80°C long term

Size: 100 µg of protein is supplied in sodium phosphate buffer containing sodium chloride and 5% glycerol, at a concentration of 1.624 mg/mL (for Lot# 1710006).

Relevance: This protein may be used in functional PVL toxicity assays in combination with LukS-PV, or as a control protein in ELISA assays or Western blotting when detecting LukF in PVL (+) strains of S. aureus.

Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution; internal QC demonstrates detection of 100 ng of LukF-PV protein when detected with antiPVL LukF polyclonal antibody (cat# 0312-002) in Western blotting.

PVL Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukS-PV (cat# 0540- 001) in a concentration range of 1-100 nM.