Recombinant Staphylococcus aureus Gamma Hemolysin A (Hlg A), Tag-Free - 100ug

Description: Purified, Staphylococcus aureus recombinant Gamma Hemolysin A (rHlg A). The rHlg A (tag free) is expressed in E. coli and purified by FPLC. The theoretical molecular weight of the protein is 34,956 Daltons.

Storage: 2-3 weeks at -20°C, -80°C long term

Size: 100 ug of protein is supplied in PBS plus 5% glycerol, at a concentration of 2.176 mg/mL (for Lot# 1805006). Protein demonstrates a molecular weight of approximately 35 kDa.

Endotoxin: 3.077 Endotoxin Units/mg (for Lot# 1805006)

Relevance: This protein may be used in functional toxicity assays in combination with rHlg B, or as a control protein in ELISA assays or Western blotting when detecting toxins produced by different strains of S. aureus.

Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution; internal QC demonstrates detection of 50 ng of rHlg A protein using anti-PVL LukS polyclonal antibody (cat# 04-0009) in Western blotting.

Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with rHlg B.