pGEX-4T-3 Plasmid - 2 ug
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    pGEX-4T-3 Plasmid Information

    Alias: pgex4t3; pgex4t-3

    TAC: promoter

    Replicon: pBR322

    Plasmid classification: Escherichia coli vector; pGEX series expression plasmid

    Plasmid size: 4968 BP

    Plasmid Tags: n-gst, n-thrombin

    Prokaryotic resistance: amp

    Clone strain: dh5a

    Culture conditions: 37 degrees

    Expression host: E.coli BL21 (DE3)

    Culture conditions: 37 ℃, aerobic, lb

    Induction method: IPTG or lactose and its analogues

    5 'sequencing primer: pgex5: gggctggcaagccacgttgtggtg

    3 'sequencing primer: pgex3: ccggaggtgcatgtcagagg

    Note: GST affinity column can be used to purify recombinant protein

    Plasmid host: Escherichia coli

    Purpose of plasmid: protein expression

    Fragment type: ORF

    Fragment species: empty bodies

    Prokaryotic resistance: amp

    pGEX-4T-3 Plasmid Description

    pGEX-4T-3 plasmid is a 4968 bp E.coli Expression Vector, which can be connected to the target gene through the enzyme cutting site at MCS, and tac promoter starts GST promoting labeling and target gene fusion expression.