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| Product name: | Human Holo TC(Holotranscobalamin) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | Holo-TC |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 0.047 ng/mL |
| Standard: | 10 ng/mL |
| Detection Range: | 0.16-10 ng/mL |
| Sample Type: | serum, plasma and other biological fluids |
| Assay Length: | 2.5h |
| Research Area: | Metabolic pathway;Nutrition metabolism; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Holo TC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Holo TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Holo TC in the samples is then determined by comparing the OD of the samples to the standard curve. |