Mouse Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit - 48 wells plate

Species Reactivity: Mouse

Specificity: This assay has high sensitivity for detection of HIF2a. 100% cross-reactivity of HIF2a was observed among mouse and rat.

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Quantitative Sandwich

Detection Range: 0.156-10ng/mL

Sensitivity: < 0.057ng/mL

Intra-assay Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HIF2a were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Inter-assay Precision:

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HIF2a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.