MIA-PACA-2 cell line - 1 vial

The MIA PaCa-2 cell line was established from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male in 1975 by A. Yunis, et al. These cells are described to express human colony stimulating factor, subclass I (CSF-I) and plasminogen activator. The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%. Sensitive to L-Asparaginase.

Species: Homo sapiens (human)

Tissue of Origin: Pancreas

Recommended Medium: DMEM + 10% h.i.FBS

Culture Properties: monolayer with floating cells

Morphology: Epithelial-like

STR DNA Profile:

Amelogenin: X

CSF1PO: 10

D13S317: 12,13

D16S539: 10,13

D5S818: 12,13

D7S820: 12,13

THO1: 9,10

TPOX: 9

vWA: 15

Karyotype: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.

Applications: The MIA PaCa-2 cell line can be used as tumourigenicity and enzymatic studies.

Disease: Carcinoma

Quality Control: Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping: Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen