PNPP (Para-nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.
Description | PNPP (Para-nitrophenyl phosphate) disodium is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays. |
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In Vitro | Phosphatases catalyze the hydrolysis of PNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. PNPP disodium Related Antibodies
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Molecular Weight | 263.05 |
Formula | C6H4NNa2O6P |
CAS No. | 4264-83-9 |
Appearance | Solid |
Color | White to yellow |
SMILES | O=P(O[Na])(O[Na])OC1=CC=C([N+]([O-])=O)C=C1 |
Shipping | Room temperature in continental US; may vary elsewhere. |
Storage | 4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |