Human EMR3 ELISA kit - 96-wells plate
Gene Name: EMR3
Detect Range: 78-5000 pg/mL
Target Protein Name:Human EMR3
Alternative Name: EMR3,Human EMR3
Sample type: serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type: Sandwich ELISA Kit
ELISA Test Principle:
The microtiter plate provided in Human EMR3 ELISA KIT has been pre-coated with an Human EMR3 antibody specific to Human EMR3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human EMR3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human EMR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human EMR3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!