Recombinant Human Latent TGF Beta-1 and GARP Complex Protein (N-6xHis, C-3xStrep) - 1 mg

Recombinant Human Latent TGF Beta-1 & GARP Complex Protein is produced in a Mammalian expression system and the target gene encoding Leu30-Ser390 & His20-Asn627 is expressed with a 6xHis tag at the N-terminus and, a 3xStrep tag at the C-terminus.

Accession: P01137 & Q14392

Gene names: TGF Beta-1, GARP

Mol Mass: 42.1 & 71.0 KDa

APMol Mass: 10-14&35-50 & 85-95 KDa, reducing conditions

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, 5%Trehalose, 5% Mannitol, 0.05% Tween 80, pH 7.4.

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Purity-SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Reconstitution:

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

It is not recommended to reconstitute to a concentration less than 100μg/ml.

Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage:

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.

Reconstituted protein solution can be stored at 2-8°C for 2-7 days.

Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Shipping:

The product is shipped at ambient temperature.

Upon receipt, store it immediately at the temperature listed below.

Background:

Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. Leucine Rich Repeat Containing 32 (LRRC32), also known as Glycoprotein A Repetitions Predominant (GARP), has been postulated as a novel surface marker of activated T(regs). LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation.

For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.