M1839
Clone 1E4
This clone was derived from hybridization of SP2/0-Ag14 myeloma cells with
spleen cells of a mouse immunized with the peptide QWNKPSKPKTN#
(corresponding to the bovine PrP AA sequence 108-119; #=amidated carboxyterminus) coupled to KLH at its N-terminal end via a CG-AA linker.
Isotype IgG1κ

Assay type: Qualitative Sandwich Lateral Flow
Sensitivity: 98.3%
Specificity: 99.3%
Assay time: 10 minutes
Sample types: Trachea and Cloacal orifice secretions

Assay type: Qualitative Sandwich Lateral Flow
Sensitivity: 98.7%
Specificity: 99.7%
Assay time: 10 minutes
Sample types: Trachea and Cloacal orifice secretions

Assay type: Qualitative Sandwich Lateral Flow
Sensitivity: 98.3%
Specificity: 99.3%
Assay time: 10 minutes
Sample types: Trachea and Cloacal orifice secretions

Sequence:
TACTGTGACGAGGACGGATC

Sequence:
CCTCAAAAGCAGATTCGGAGT

Sequence:
CACGAGCAAGGAACTACCGATGTTGA

Sequence:
CCAAGGACCTTCAACGTACGAGGTCA

Sequence:
TCAACAAGGTACTCTATATGGCTACC

Sequence:
CTACCGAGCAGAACCTTCTTCACCAT

Assay type: Sandwich ELISA
Assay principle: Coated antigen PA83 -> Anti-PA83 IgG bind to the coated antigen -> HRP-labeled protein G binds to the IgG
Standard/Control: Rabbit Anti-PA83 IgG
*Note: The interaction between Protein G and IgG is not species specific

Protein sequence:
GAFNYLTLLQSGISLAGSFVPGGTFVAPIVNMVIGWLWPHKNKTADTENLIKLIDEEIQKQLNKALLDQDRNNWTSFLESIFDTSATVSNAIIDAQWSGTVD
TTNRQQKTPTTSDYLNVVGKFDSADSSIITNENQIMNGNFDVAAAPYFVIGATLRLSLYQSYIKFCNSWIDAVGFSTNDANTQKANLARTKLTMRTTINEYT
QRVMKVFKDSKNMPTIGTNKFSVDAYNVYVKGMTLNVLDMVAIWSSLYP