| Product name: | Hyp (Hydroxyproline) ELISA Kit |
| Reactivity: | General |
| Alternative Names: | (2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N) |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 26.3 ng/mL |
| Standard: | 5000 ng/mL |
| Range: | 78.13-5000 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Metabolic pathway;Nutrition metabolism; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hydroxyproline(Hyp) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hydroxyproline(Hyp). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxyproline(Hyp) in the samples is then determined by comparing the OD of the samples to the standard curve. |